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resistant e coli strains atcc 25922  (ATCC)


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    ATCC resistant e coli strains atcc 25922
    Resistant E Coli Strains Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 53956 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Flow chart of colistin resistant strain induction assay. Colistin concentration was started at one-half MIC of E. coli ATCC 25922, doubling every 24 hours until bacterial growth was completely inhibited. Cultures were plated on MH agar plates and the resistant colony was named E. coli ATCC 25922-R.

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Flow chart of colistin resistant strain induction assay. Colistin concentration was started at one-half MIC of E. coli ATCC 25922, doubling every 24 hours until bacterial growth was completely inhibited. Cultures were plated on MH agar plates and the resistant colony was named E. coli ATCC 25922-R.

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Concentration Assay

    Point mutations in pmrB and relA of E. coli ATCC 25922-R (A) and gene expressions in E. coli ATCC 25922-R by quantitative RT-PCR (B) . Relative expressions of genes were determined using 2 −ΔΔCt method. Error bars represent the standard deviations of three biological repeats.

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Point mutations in pmrB and relA of E. coli ATCC 25922-R (A) and gene expressions in E. coli ATCC 25922-R by quantitative RT-PCR (B) . Relative expressions of genes were determined using 2 −ΔΔCt method. Error bars represent the standard deviations of three biological repeats.

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Quantitative RT-PCR

    Difference in gene expression profile between the two groups. Sample A E. coli ATCC 25922. Sample B E. coli ATCC 25922-R. Sample C E. coli ATCC 25922-R grown with 2 mg/L colistin. The red and blue bars represent up- and down-regulated genes. Number labels represent the number of genes in each group.

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Difference in gene expression profile between the two groups. Sample A E. coli ATCC 25922. Sample B E. coli ATCC 25922-R. Sample C E. coli ATCC 25922-R grown with 2 mg/L colistin. The red and blue bars represent up- and down-regulated genes. Number labels represent the number of genes in each group.

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Gene Expression

    Heat map of hierarchical clustering in differentially expressed genes in the groups. (A): E coli ATCC 25922 (sample A) and E. coli ATCC 25922-R (sample B); (B): E coli ATCC 25922-R (sample B) and E. coli ATCC 25922-R grown with 2 mg/L colistin (sample C). Blue represents down-regulated expression and red represents up-regulated expression, respectively, relative to that of the reference culture.

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Heat map of hierarchical clustering in differentially expressed genes in the groups. (A): E coli ATCC 25922 (sample A) and E. coli ATCC 25922-R (sample B); (B): E coli ATCC 25922-R (sample B) and E. coli ATCC 25922-R grown with 2 mg/L colistin (sample C). Blue represents down-regulated expression and red represents up-regulated expression, respectively, relative to that of the reference culture.

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Expressing

    Top Ten Up- and Down-Regulated E. coli ATCC 25922-R (Sample B) Genes in Comparison with E. coli ATCC 25922 (Sample A)

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Top Ten Up- and Down-Regulated E. coli ATCC 25922-R (Sample B) Genes in Comparison with E. coli ATCC 25922 (Sample A)

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Comparison

    Top Ten Up-Regulated Genes in E. coli ATCC 25922-R Resistant Treated with 2mg/L Colistin (Sample C)

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Top Ten Up-Regulated Genes in E. coli ATCC 25922-R Resistant Treated with 2mg/L Colistin (Sample C)

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques:

    KEGG pathway enrichment analysis of differentially expressed genes between the two groups. (A): Pathway enrichment genes for sample A ( E. coli ATCC 25922) and sample B ( E. coli ATCC 25922-R). (B): Pathway enrichment genes for sample C ( E. coli ATCC 25922-R grown with 2 mg/L colistin) and sample B ( E. coli ATCC 25922-R).

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: KEGG pathway enrichment analysis of differentially expressed genes between the two groups. (A): Pathway enrichment genes for sample A ( E. coli ATCC 25922) and sample B ( E. coli ATCC 25922-R). (B): Pathway enrichment genes for sample C ( E. coli ATCC 25922-R grown with 2 mg/L colistin) and sample B ( E. coli ATCC 25922-R).

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques:

    Gene expressions of TCS EvgS/EvgA in E. coli ATCC 25922 and E. coli ATCC 25922-R in comparison with their counterparts by quantitative RT-qPCR. Relative expressions of genes were determined using 2 −ΔΔCt method. Error bars represent the standard deviations of three biological repeats.

    Journal: Infection and Drug Resistance

    Article Title: Genomic and Transcriptomic Analysis of Colistin-Susceptible and Colistin-Resistant Isolates Identify Two-Component System EvgS/EvgA Associated with Colistin Resistance in Escherichia coli

    doi: 10.2147/IDR.S316963

    Figure Lengend Snippet: Gene expressions of TCS EvgS/EvgA in E. coli ATCC 25922 and E. coli ATCC 25922-R in comparison with their counterparts by quantitative RT-qPCR. Relative expressions of genes were determined using 2 −ΔΔCt method. Error bars represent the standard deviations of three biological repeats.

    Article Snippet: E. coli ATCC 25922 was treated with colistin to obtain colistin resistant strain E. coli ATCC 25922-R as described in materials and methods.

    Techniques: Comparison, Quantitative RT-PCR

    Genomic variation and resistance of E. coli mutant populations serial passaged for 7 d either with or without LepB inhibitor, G0775

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Single-molecule nanopore sequencing reveals extreme target copy number heterogeneity in arylomycin-resistant mutants

    doi: 10.1073/pnas.2021958118

    Figure Lengend Snippet: Genomic variation and resistance of E. coli mutant populations serial passaged for 7 d either with or without LepB inhibitor, G0775

    Article Snippet: During the chemical optimization and development of a bacterial type I signal peptidase inhibitor , candidate molecules were evaluated for potency and resistance frequency against E. coli ATCC strain 25922 and A. baumannii ATCC strain 17978.

    Techniques: Mutagenesis